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Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
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Chimica
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JoVE Journal Chimica
Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

DOI:
10.3791/58771-v

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08:30 min

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January 19, 2019

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,
1Molecular Diagnostics, Centre for Health and Bioresources,AIT Austrian Institute of Technology GmbH

Capitoli

  • 00:04Titolo
  • 01:12Polyplex Formation
  • 02:16Agarose Gel Electrophoresis
  • 03:12Stability of Polyplexes Towards DNase I
  • 04:36Testing the Addressability of Horseradish Peroxidase Enzyme (HRP)-functionalized DNA Origami (HRP-NR) Upon Polycation Coating
  • 05:44Results: Characterization of DNA Origami Polyplexes
  • 07:42Conclusion

Summary

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Here, a protocol for the protection of DNA origami nanostructures in Mg-depleted and nuclease-rich media using natural cationic polysaccharide chitosan and synthetic linear polyethyleneimine (LPEI) coatings is presented.

Tags

DNA OrigamiNanostructuresGene TherapyPolycation CoatingEnzymatic DegradationCellular UptakeGene DeliveryAgarose Gel Electrophoresis

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