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Identificatie van muis en menselijk antilichaam Repertoires door Next-Generation Sequencing
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
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Identificatie van muis en menselijk antilichaam Repertoires door Next-Generation Sequencing

DOI:
10.3791/58804-v

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08:51 min

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March 15, 2019

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, , , , , , , , , , , ,
1Graduate School of Life and Environmental Sciences,University of Tsukuba, 2Center for Influenza Virus Research,National Institute of Infectious Diseases, 3School of Science and Technology,Kwansei Gakuin University, 4Department of Pathology,National Institute of Infectious Diseases, 5Division of Infectious Diseases Pathology, Department of Global Infectious Diseases,Tohoku University Graduate School of Medicine, 6Division of Molecular Pathobiology, Research Center for Zoonosis Control,Hokkaido University, 7Division of Collaboration and Education, Research Center for Zoonosis Control,Hokkaido University, 8Global Station for Zoonosis Control, GI-CoRE,Hokkaido University, 9Department of Immunology,National Institute of Infectious Diseases, 10Faculty of Life and Environmental Sciences,University of Tsukuba

Capitoli

  • 00:04Titolo
  • 01:23Nucleic Acid Isolation from Immune Cells and Tissues
  • 03:04cDNA Synthesis and PCR Amplification
  • 04:58NGS Sequencing of Libraries
  • 05:33Results: Identification of Mouse and Human Antibody Repertoires
  • 08:11Conclusion

Summary

Traduzione automatica

Traduzione automatica

We beschrijven hier protocollen voor de analyse en visualisatie van de structuur en de Grondwet van hele antilichaam repertoires. Het gaat hierbij om de overname van grote sequenties van antilichaam RNA met behulp van de volgende generatie sequencing.

Tags

Keywords: Antibody RepertoireNext-generation SequencingB Cell RepertoireImmune ResponseVaccine EfficacyImmunoglobulin AmplificationRNA Extraction

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