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Isolation and Culture of Embryonic Mouse Neural Stem Cells
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JoVE Journal Neuroscienze
Isolation and Culture of Embryonic Mouse Neural Stem Cells

Isolation and Culture of Embryonic Mouse Neural Stem Cells

DOI:
10.3791/58874-v

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09:04 min

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November 11, 2018

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, , , , , , ,
1Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology,ACECR, 2Department of Biology, Faculty of Basic Sciences,Islamic Azad University, 3Department of Biology,ACECR Institute of Higher Education

Capitoli

  • 00:00Titolo
  • 01:18Animal Surgery and Micro-Dissection
  • 02:51Bending the Tip of the Needle
  • 03:12Isolation of E13 Mouse Brain and Micro-Dissection
  • 06:32Trypsination and Passaging of Neurospheres
  • 07:46Representative Results
  • 08:42Conclusion

Summary

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Here, we introduce a novel microsurgical technique for the isolation of neural stem cells from the E13 mouse embryo ganglionic eminence.

Tags

Keywords: Embryonic MouseNeural Stem CellIsolationCultureGanglionic EminenceMicro-dissectionSingle Cell SuspensionHEPES MEM BufferDissociationSuspension CultureNerve Cells

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