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微电极阵列接口微流体研究神经元通信和轴向信号传播

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Bioingegneria
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JoVE Journal Bioingegneria

Interfacing Microfluidics with Microelectrode Arrays for Studying Neuronal Communication and Axonal Signal Propagation

Please note that all translations are automatically generated. Click here for the English version.

微电极阵列接口微流体研究神经元通信和轴向信号传播

DOI:

10.3791/58878-v

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11:27 min

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December 08, 2018

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Cátia D.F. Lopes², José C. Mateus^2,3, Paulo Aguiar²

¹i3S – Instituto de Investigação e Inovação em Saúde,Universidade do Porto, ²INEB – Instituto de Engenharia Biomédica,Universidade do Porto, ³ICBAS – Instituto de Ciências Biomédicas Abel Salazar,Universidade do Porto

Capitoli

00:00Titolo
01:37Microfluidic and MEA Devices Preparation
03:55Prefrontal Cortex Dissection
05:01Cortical Neurons Dissociation and Culture
08:03Recording and Data Analysis
09:58Representative Results
11:00Conclusion

Summary

Traduzione automatica

该协议旨在说明如何将体外微电极阵列与微流体器件结合起来, 研究神经元培养中的动作电位传递。数据分析, 即传播动作电位的检测和表征, 是使用一种新的先进的、但方便用户和免费提供的计算工具进行的。

Tags

Microfluidics Microelectrode Arrays Neuronal Communication Axonal Signal Propagation Neural Circuits Neural Coding Neural Decoding Sensory Neurons Cell Culture Data Analysis Computational Tools PEI Coating MEA Preparation

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