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大肠杆菌无细胞蛋白合成: 一种强大、灵活和可访问的平台技术的协议
JoVE Journal
Chimica
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JoVE Journal Chimica
Escherichia coli-Based Cell-Free Protein Synthesis: Protocols for a robust, flexible, and accessible platform technology
DOI:

09:45 min

February 25, 2019

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Capitoli

  • 00:04Titolo
  • 00:50Cell Growth
  • 03:30Crude Cell Extract Preparation
  • 05:30Cell-Free Protein Synthesis Batch Format Reactions
  • 06:25Quantification of the Reporter Protein, [sfGFP]
  • 07:24Results: Modification and Optimization of the Cell-Free Platform Conditions
  • 09:01Conclusion

Summary

Traduzione automatica

该协议详细介绍了在4天或更短的时间内生成大肠杆菌基细胞提取物并实施体外蛋白质合成反应所需的步骤、成本和设备。为了将这一平台的灵活性用于广泛的应用, 我们讨论了可以调整和优化的反应条件。

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