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一种用于蛋白质溶解活性的荧光肽裂解检测: 冠状病毒穗蛋白活化的应用
JoVE Journal
Biochimica
This content is Free Access.
JoVE Journal Biochimica
A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation
DOI:

07:53 min

January 09, 2019

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Capitoli

  • 00:04Titolo
  • 00:31Designing and Preparing the Peptides
  • 02:06Preparing the Fluorescence Plate Reader
  • 03:11Preparing the Assay
  • 04:30Data Analysis
  • 05:37Results: Mutations in the Cleavage Site Alter Activity of Furin and Cleavage Efficiency
  • 07:26Conclusion

Summary

Traduzione automatica

我们提出了一个氟原肽裂解检测, 允许快速筛选蛋白酶的蛋白溶解活性肽代表病毒融合肽的裂解部位。这种方法也可以用于蛋白质序列中的任何其他氨基酸母题, 以测试蛋白酶活性。

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