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A New Portable In Vitro Exposure Cassette for Aerosol Sampling
 

A New Portable In Vitro Exposure Cassette for Aerosol Sampling

Article DOI: 10.3791/58916-v 07:01 min February 22nd, 2019
February 22nd, 2019

Capitoli

Riepilogo

Here, we present a protocol to perform portable cellular aerosol exposures and measure cellular response. The method uses cells, grown at the air-liquid interface, mimicking in vivo physiology. Cellular response to copper nanoparticle aerosols was observed as oxidative stress through reactive oxygen species generation and cytotoxicity as lactate dehydrogenase release.

Tags

Keywords: In Vitro Exposure Aerosol Sampling PIVEC Portable Exposure System Air Quality Monitoring Lung Cell Culture Dry Dispersal System Copper Nanoparticles HEPA Filter Vacuum Generator Air-liquid Interface Cell Culture Insert
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