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Immunology and Infection

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JoVE Journal Immunology and Infection
Induction of Mouse Lung Injury by Endotracheal Injection of Bleomycin
 

Induction of Mouse Lung Injury by Endotracheal Injection of Bleomycin

Article DOI: 10.3791/58922-v • 06:52 min • April 30th, 2019
April 30th, 2019 •
*1, *2, 2, 2, 2, 3, 3, 1, 2, 2
1Servizio di Allevamento e Sperimentazione Animale, Polo ScientificoTecnologico, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS)-Istituto Nazionale Ricovero e Cura Anziani (INRCA), 2Dipartimento di Scienze Cliniche e Molecolari, Università Politecnica delle Marche, 3UOS Centro di Terapia Cellulare "G. Lanzani", Azienda Socio Sanitaria Territoriale (ASST) Papa Giovanni XXIII
* These authors contributed equally

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Here we present an effective method to investigate the antifibrotic activity of intravenously infused human mesenchymal stromal cells obtained from the whole umbilical cord following the induction of lung injury by an endotracheal injection of bleomycin in C57BL/6 mice. This protocol can be easily extended to the preclinical testing of other therapeutics.

Tags

Mouse Lung Injury Endotracheal Injection Bleomycin Inflammatory Response Fibrotic Response Anti-inflammatory Therapy Anti-fibrotic Therapy Systemic Therapy Tail Vein Infusion Safe And Robust Induction Lung Inflammation Lung Fibrosis Veterinary Doctor Surgical Platform Anesthetized Mouse Hyperextend The Neck Shave The Throat Incision Sternohyoid Muscle Trachea Exteriorization
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