Using the Protozoan <em>Paramecium caudatum</em> as a Vehicle for Food-borne Infections in Zebrafish Larvae

Erika Flores; Laurel Thompson; Natalie Sirisaengtaksin; Anh Trinh Nguyen; Abigail Ballard; Anne-Marie Krachler

doi:10.3791/58949

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Immunologia e infezioni

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Using the Protozoan Paramecium caudatum as a Vehicle for Food-borne Infections in Zebrafish Larvae

JoVE Journal
Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Using the Protozoan Paramecium caudatum as a Vehicle for Food-borne Infections in Zebrafish Larvae

Using the Protozoan Paramecium caudatum as a Vehicle for Food-borne Infections in Zebrafish Larvae

DOI:

10.3791/58949-v

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07:49 min

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January 07, 2019

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Erika Flores, Laurel Thompson, Natalie Sirisaengtaksin, Anh Trinh Nguyen, Abigail Ballard, Anne-Marie Krachler

¹McGovern Medical School, Department of Microbiology and Molecular Genetics,University of Texas Health Science Center at Houston

Capitoli

00:04Titolo
00:40Paramecia Growth and Maintenance and Bacterial Dose Determination
02:23Paramecia Preparation for Food-borne Infection
04:40Zebrafish Food-borne Infection and Pre-rate Determination
06:30Results: Representative Zebrafish Bacterial Colonization Post Paramecia Consumption
07:28Risultati

Summary

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Zebrafish (Danio rerio) are becoming a widely-used vertebrate animal model for microbial colonization and pathogenesis. This protocol describes the use of the protozoan Paramecium caudatum as a vehicle for food-borne infection in zebrafish larvae. P. caudatum readily internalizes bacteria and get taken up by larval zebrafish through natural preying behavior.

Using the Protozoan Paramecium caudatum as a Vehicle for Food-borne Infections in Zebrafish Larvae

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