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一种碘-黄色荧光荧光蛋白-缝隙--细胞间通信方法
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Biologia
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JoVE Journal Biologia
An Iodide-Yellow Fluorescent Protein-Gap Junction-Intercellular Communication Assay
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一种碘-黄色荧光荧光蛋白-缝隙--细胞间通信方法

DOI:
10.3791/58966-v

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09:47 min

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February 01, 2019

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1College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences,Yonsei University

Capitoli

  • 00:04Titolo
  • 01:05Generation of LN215-YFPQL and LN215-I” Cells by Lentiviral Transduction
  • 03:14Plating the LN215-YFPQL and LN215-I‐ Cells
  • 04:42Conducting the I-YFP GJIC Assay
  • 07:37Results: Analysis of the Iodine-Yellow Fluorescent Protein-Gap Junction Intercellular Communication Assay
  • 08:15Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里, 我们提出了一种新的缝隙结细胞间通信检测的协议, 旨在为药物发现和毒理学评估的间隙结调节化学品的高通量筛选。

Tags

Iodide-YFPGap JunctionIntercellular CommunicationHigh-throughput ScreeningAssayLN215 CellsLentivirusPuromycinCell Culture

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