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Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy
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JoVE Journal Genetica
Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy

Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy

DOI:
10.3791/58971-v

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10:16 min

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January 25, 2019

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, , , ,
1Department of Biomedical Engineering, School of Engineering, School of Medicine,University of Alabama at Birmingham

Capitoli

  • 00:04Titolo
  • 00:50Comet Assay
  • 03:19DNA Damage Response
  • 06:12Counting of the DNA Damage Response (DDR) Foci
  • 08:11Results: DNA Damage Response in Human iPS Cells and iPS-derived Cardiomyocytes
  • 09:43Conclusion

Summary

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Here, we provide a detailed description of an experimental setup for an analysis of the assessment of DNA integrity in stem cells prior to cell transplantation.

Tags

DNA IntegrityStem Cell Quality AssessmentComet AssayDNA DamageRegenerative TherapyCardiomyopathyInduced Pluripotent Stem CellsReactive Oxygen SpeciesCell TransplantationDNA-RNA Free WaterAgaroseComet SlideLysis BufferAlkaline SolutionElectrophoresisEthanol

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