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心脏细胞治疗中干细胞 dna 完整性的评估

JoVE Journal
Genetica

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JoVE Journal Genetica

Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy

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心脏细胞治疗中干细胞 dna 完整性的评估

DOI:

10.3791/58971-v

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10:16 min

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January 25, 2019

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Jessica M. Miller*¹, Nikhil Maneesh Mardhekar*¹, Vasanthi Rajasekaran, Jianyi Zhang, Ram Kannappan

¹Department of Biomedical Engineering, School of Engineering, School of Medicine,University of Alabama at Birmingham

Capitoli

00:04Titolo
00:50Comet Assay
03:19DNA Damage Response
06:12Counting of the DNA Damage Response (DDR) Foci
08:11Results: DNA Damage Response in Human iPS Cells and iPS-derived Cardiomyocytes
09:43Conclusion

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在这里, 我们提供了一个实验设置的详细描述, 用于分析细胞移植前干细胞 dna 完整性的评估。

Tags

DNA Integrity Stem Cell Quality Assessment Comet Assay DNA Damage Regenerative Therapy Cardiomyopathy Induced Pluripotent Stem Cells Reactive Oxygen Species Cell Transplantation DNA-RNA Free Water Agarose Comet Slide Lysis Buffer Alkaline Solution Electrophoresis Ethanol

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