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基于细菌选择和生长的盘状细胞基因工程
JoVE Journal
Genetica
This content is Free Access.
JoVE Journal Genetica
Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria
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基于细菌选择和生长的盘状细胞基因工程

DOI:
10.3791/58981-v

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06:08 min

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January 25, 2019

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, , , , , , ,
1MRC Laboratory of Molecular Biology, 2Department of Molecular and Cell Biology,University of Connecticut, 3Cancer Research UK Beatson Institute Glasgow, 4MRC Laboratory for Molecular Cell Biology,University College London, 5Department of Cell and Developmental Biology,University College London

Capitoli

  • 00:04Titolo
  • 00:54Preparation of Cells and Materials
  • 02:30Transfection of Dictyostelium Cells Based on Bacterial Selectionin Protocol
  • 04:15Results: Dual Reporter Extrachromosomal Plasmid System Demonstrated
  • 05:37Conclusion

Summary

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盘状双翅目是研究细胞迁移、内吞和发育等复杂细胞过程的常用模型生物。生物体的效用取决于基因操纵的可行性。在这里, 我们提出了方法, 以转移牙菌细胞, 克服现有的限制培养细胞在液体介质。

Tags

Keywords: Dictyostelium DiscoideumGenetic EngineeringBacterial SelectionGrowth On BacteriaExochromosomal Plasmid TransfectionMicroscopyKlebsiella AerogenesSorensen BufferLB MediumOptical DensityH40 Electroporation BufferSM MediumAgar PlateCell TransferCentrifugationDNA Transformation

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