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Designe porøs silicium film som bærere af Nerve vækst faktor
JoVE Journal
Bioingegneria
This content is Free Access.
JoVE Journal Bioingegneria
Designing Porous Silicon Films as Carriers of Nerve Growth Factor
DOI:

10:12 min

January 25, 2019

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Capitoli

  • 00:04Titolo
  • 00:57Oxidized Porous Silicon (PSiO2) Carrier Fabrication
  • 02:10Nerve Growth Factor (NGF) Loading and Quantification and In Vitro NGF Release from PSiO2 Carriers
  • 04:42Cell Viability and Growth in the Presence of NGF-Loaded PSiO2 Carriers
  • 06:00Cell Differentiation Analysis
  • 07:27Results: Representative Effects of NGF-Loaded PSiO2 Carriers on Neuronal Growth and Development
  • 08:58Conclusion

Summary

Traduzione automatica

Vi præsenterer her, en protokol for at designe og fremstille nanostrukturerede porøs silicium (PSi) film som nedbrydeligt bærere af den nerve vækstfaktor (NGF). Neuronal differentiering og udvækst af PC12 celler og mus dorsalrods ganglion (DRG) neuroner er karakteriseret ved behandling med NGF-loaded PSi luftfartsselskaber.

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