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Measuring Bone Remodeling and Recreating the Tumor-Bone Microenvironment Using Calvaria Co-culture and Histomorphometry
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JoVE Journal Ricerca sul cancro
Measuring Bone Remodeling and Recreating the Tumor-Bone Microenvironment Using Calvaria Co-culture and Histomorphometry

Measuring Bone Remodeling and Recreating the Tumor-Bone Microenvironment Using Calvaria Co-culture and Histomorphometry

DOI:
10.3791/59028-v

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08:24 min

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March 14, 2020

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, ,
1Biomedical Innovation Department,Center for Scientific Research and Higher Education at Ensenada

Capitoli

  • 00:00Introduction
  • 00:44Calvarial Dissection
  • 03:03Calvaria and Cancer Cells Co-culture
  • 04:19Tissue Processing
  • 05:49Risultati
  • 07:36Conclusion

Summary

Traduzione automatica

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The ex vivo culture of bone explants can be a valuable tool for the study of bone physiology and the potential evaluation of drugs in bone remodeling and bone diseases. The presented protocol describes the preparation and culture of calvarias isolated from newborn mice skulls, as well as its applications.

Tags

Bone RemodelingCalvaria Co-cultureTumor-bone MicroenvironmentHistomorphometryEx Vivo ModelMouse CalvariaOrgan CultureBone RegenerationCancer CellsBone-tumor Microenvironment

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