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利用卡尔瓦里亚共同培养和教条法测量骨重塑和再造肿瘤-骨微环境

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Measuring Bone Remodeling and Recreating the Tumor-Bone Microenvironment Using Calvaria Co-culture and Histomorphometry

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利用卡尔瓦里亚共同培养和教条法测量骨重塑和再造肿瘤-骨微环境

DOI:

10.3791/59028-v

•

08:24 min

•

March 14, 2020

•

Cinthia N. Cuero, Brenda Iduarte, Patricia Juárez

¹Biomedical Innovation Department,Center for Scientific Research and Higher Education at Ensenada

Capitoli

00:00Introduction
00:44Calvarial Dissection
03:03Calvaria and Cancer Cells Co-culture
04:19Tissue Processing
05:49Risultati
07:36Conclusion

Summary

Traduzione automatica

骨外植物的外生培养是骨生理学研究的宝贵工具，是骨改和骨病药物的潜在评价。提交的协议描述了从新生小鼠头骨分离的钙瓦里亚的制备和培养及其应用。

Tags

Bone Remodeling Calvaria Co-culture Tumor-bone Microenvironment Histomorphometry Ex Vivo Model Mouse Calvaria Organ Culture Bone Regeneration Cancer Cells Bone-tumor Microenvironment

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