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Handling and Assessment of Human Primary Prostate Organoid Culture
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Handling and Assessment of Human Primary Prostate Organoid Culture

Handling and Assessment of Human Primary Prostate Organoid Culture

DOI:
10.3791/59051-v

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08:45 min

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January 17, 2019

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, , , ,
1Department of Pathology,University of Illinois at Chicago, 2Departments of Urology, Physiology, and Biophysics,University of Illinois at Chicago, 3University of Illinois Cancer Center

Capitoli

  • 00:04Titolo
  • 01:04Collection of Organoids from Matrix Gel
  • 02:09Formalin Fixation and Paraffin Embedding of Organoids for Histological Endpoints
  • 04:00Sectioning the Formalin Fixed Paraffin Embedded (FFPE) Histology Gel Blocks
  • 05:04Whole-mounting of Organoids for Immunofluorescent Staining
  • 06:35Results: Analysis of Human Primary Prostate Organoids
  • 07:56Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present a protocol to guide human primary prostate organoid handling then suggest endpoints to assess phenotype. Seeding, culture maintenance, recovery from matrix gel, morphologic quantification, embedding and sectioning, FFPE sectioning, whole-mount staining, and application of commercial assays are described.

Tags

Keywords: Prostate OrganoidsIn Vitro CulturePrimary CellsMatrix GelNeutral ProteaseHistology EmbeddingPrecision MedicineDisease Modeling

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