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Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota
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JoVE Journal Bioingegneria
Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota

Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota

DOI:
10.3791/59054-v

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06:23 min

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February 15, 2019

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, , , , ,
1Dairy and Functional Foods Research Unit, Eastern Regional Research Center,Agricultural Research Service, United States Department of Agriculture, 2ProDigest, 3Division of Gastroenterology, Hepatology, and Nutrition,Children’s Hospital of Philadelphia

Capitoli

  • 00:04Titolo
  • 00:36System Inoculation
  • 01:38Luminal Fluid and Mucosal Sample Harvest
  • 03:30Daily Feeding Cycles
  • 04:27Results: Representative Stable Community Comparison Between the In Vitro System and the Fecal Inoculum
  • 05:52Conclusion

Summary

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Traduzione automatica

Here, we present a protocol for culturing the gut microbiota of the colon in vitro, using a series of bioreactors that simulate the physiological conditions of the gastro intestinal tract.

Tags

Keywords: In Vitro CulturingGut MicrobiotaColonDefined MediumMucinFecal HomogenateBioreactorPH ControlLuminal SampleMucosal SampleDNA AnalysisShort-chain Fatty Acids

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