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由可逆连接的运动素提供动力的分子穿梭机的组装
JoVE Journal
Ingegneria
This content is Free Access.
JoVE Journal Ingegneria
Assembling Molecular Shuttles Powered by Reversibly Attached Kinesins
DOI:

08:04 min

January 26, 2019

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Capitoli

  • 00:04Titolo
  • 00:37Microtubule Preparation
  • 02:02Motility Solution
  • 03:06Assembling the Flow Cells
  • 05:08Flowing the Solutions into a Flow Cell
  • 05:45Imaging a Flow Cell
  • 06:41Results: Analysis of Assembled Molecular Shuttles
  • 07:31Conclusion

Summary

Traduzione automatica

我们提出了一个协议, 以建立分子航天飞机, 其中表面粘附的运动蛋白推动染料标记微管。激素与表面的弱相互作用使它们能够可逆地附着在表面上。这就创建了一个纳米系统, 该系统可显示其组件的动态组装和拆卸, 同时保留其功能。

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