Rivista
/
/
酵母作为开发功能分析研究人类P53的机箱
JoVE Journal
Ricerca sul cancro
Author Produced
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Ricerca sul cancro
Yeast As a Chassis for Developing Functional Assays to Study Human P53
DOI:

14:57 min

August 04, 2019

, , , , ,

Capitoli

  • 00:00Titolo
  • 00:54Construction of ADE2 or LUC1 Reporter Yeast Strain that Contains a Specific RE (yAFM-RE or yLFM-RE)
  • 03:06Evaluation of P53 Protein Transactivation Ability using the Qualitative Color-based ADE2 Yeast Assay
  • 04:27Evaluation of P53 Protein Transactivation Ability using the Quantitative Luminescence-based LUC1 Yeast Assay
  • 07:06Evaluation of P53 Protein Growth Inhibition using the Yeast Phenotypic Assay
  • 08:08Risultati
  • 13:51Conclusion

Summary

Traduzione automatica

这里提出的四个协议,以构造和利用酵母糖糖酶报告菌株,以研究人类P53转活化潜力,其各种癌症相关突变的影响,共同表达的相互作用蛋白,和特定小分子的影响。

Video correlati

Read Article