/
/
Uptake of New Lipid-coated Nanoparticles Containing Falcarindiol by Human Mesenchymal Stem Cells
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
1Department of Chemical Engineering, Biotechnology and Environmental Technology,University of Southern Denmark, 2Center for Single Particle Science and Engineering (SPSE), Institute for Molecular Medicine, Health Sciences,University of Southern Denmark, 3Department of Mechanical Engineering and Material Science,Duke University, 4School of Pharmacy,University of Nottingham
Capitoli
- 00:04Titolo
- 01:13Nanoparticle Synthesis by Rapid Solvent Shifting Technique
- 04:29Particle Suspension and Size Analysis using the DLS Technique
- 05:27Cell Treatment and Imaging
- 07:26Results: Uptake of Lipid-coated Nanoparticles
- 08:48Conclusion
This article describes the encapsulation of falcarindiol in lipid-coated 74 nm nanoparticles. The cellular uptake of the nanoparticles by human stem cells into lipid droplets is monitored by fluorescent and confocal imaging. Nanoparticles are fabricated by the rapid injection method of solvent shifting, and their size is measured with the dynamic light scattering technique.
