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微链 rna 的共聚焦成像和模式识别受体在负感 rna 病毒感染中的应用
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection
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微链 rna 的共聚焦成像和模式识别受体在负感 rna 病毒感染中的应用

DOI:
10.3791/59095-v

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06:44 min

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January 26, 2019

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, ,
1Department of Pathology and Institute for Human Infections and Immunity,University of Texas Medical Branch

Capitoli

  • 00:04Titolo
  • 01:05Preparation of A549 Cells and JUNV (Junn virus) Infection
  • 02:09Fixation and Immunostaining
  • 05:10Results: Distribution and Colocalization Between the Retinoic Acid Like Receptors RLRs (RIG-I and MDA-5) and dsRNA in JUNV-infected Cells
  • 05:52Conclusion

Summary

Traduzione automatica

Traduzione automatica

在 rna 病毒复制过程中产生的双链 rna 可以通过模式识别受体来识别, 从而诱导先天免疫反应。对于负感 rna 病毒, 低水平 dsrna 和 prr 之间的相互作用仍不清楚。我们开发了一种共聚焦显微镜方法, 用于在单个细胞中显示腺病毒 dsrna 和 prr。

Tags

Keywords: Confocal ImagingDouble-stranded RNAPattern Recognition ReceptorsNegative-sense RNA VirusRNA-FISHRIG-IMDA-5DsRNA AntibodyVirus InfectionInnate Immune Response

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