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Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
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Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids

DOI:
10.3791/59106-v

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11:08 min

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January 07, 2019

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, ,
1Department of Biosystems Engineering,Auburn University

Capitoli

  • 00:04Titolo
  • 00:42Setup of Bubble Column Photobioreactors
  • 02:13Cultivation of Microalgae in Bubble Column Photobioreactors
  • 04:03Harvest and Freeze Drying of Microalgal Biomass
  • 05:28Lipid Extraction Using a Modified Folch Method
  • 07:07Neutral Lipid Assay Using a Microplate Method (Adapted from Higgins et al. 201422)
  • 09:01Results: Auxenochlorella protothecoides Growth Results and Chlorella sorokiniana Neutral Lipid Data
  • 10:35Conclusion

Summary

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Here, we present a protocol to construct lab-scale bubble column photobioreactors and use them to culture microalgae. It also provides a method for the determination of the culture growth rate and neutral lipid content.

Tags

Keywords: MicroalgaeBubble Column PhotobioreactorAlgae BiofuelsWastewater TreatmentAlgae BiologyReactor SystemO-ringsCheck ValvesAir FiltersCentrifugationAlgae ConcentrationPhotobioreactors

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