Rivista
/
Bioingegneria
/
Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro
JoVE Journal
Bioingegneria
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Bioingegneria
Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro

Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro

DOI:
10.3791/59127-v

•

08:11 min

•

January 31, 2019

•

, , , , ,
1Frankfurt Initiative for Regenerative Medicine,Johann Wolfgang Goethe-University, 2J. Crayton Pruitt Family Department of Biomedical Engineering,University of Florida

Capitoli

  • 00:04Titolo
  • 00:46Construction of Electrical Stimulation Cell Culture Chamber
  • 03:22Treating MSCs with EStim
  • 05:48Results: Effect of EStim on MSC Morphology and MSC Osteogenic Marker Gene Expression
  • 07:22Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here we present a protocol for the construction of a cell culture chamber designed to expose cells to various types of electrical stimulation, and its use in treating mesenchymal stem cells to enhance osteogenic differentiation.

Tags

Keywords: Electrical Stimulation ChamberMesenchymal Stem CellsOsteogenic DifferentiationCell BehaviorPlatinum WireSilver-coated WireLEDSix-well Plate LidTerminal Block Connector

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/59127?article_type=v

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation
check_url/it/59127?article_type=v

Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model
check_url/it/59127?article_type=v

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery
check_url/it/59127?article_type=v

An In Vitro Organ Culture Model of the Murine Intervertebral Disc
check_url/it/59127?article_type=v

Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation
check_url/it/59127?article_type=v

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
check_url/it/59127?article_type=v

Simultaneous Electrical and Mechanical Stimulation to Enhance Cells’ Cardiomyogenic Potential
check_url/it/59127?article_type=v

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
check_url/it/59127?article_type=v

Silicon Nanowires and Optical Stimulation for Investigations of Intra- and Intercellular Electrical Coupling
check_url/it/59127?article_type=v

Simple, Affordable, and Modular Patterning of Cells using DNA

Read Article