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电刺激室的构建与应用, 促进间充质茎间质细胞体外成骨分化
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JoVE Journal Bioingegneria
Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro
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电刺激室的构建与应用, 促进间充质茎间质细胞体外成骨分化

DOI:
10.3791/59127-v

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08:11 min

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January 31, 2019

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, , , , ,
1Frankfurt Initiative for Regenerative Medicine,Johann Wolfgang Goethe-University, 2J. Crayton Pruitt Family Department of Biomedical Engineering,University of Florida

Capitoli

  • 00:04Titolo
  • 00:46Construction of Electrical Stimulation Cell Culture Chamber
  • 03:22Treating MSCs with EStim
  • 05:48Results: Effect of EStim on MSC Morphology and MSC Osteogenic Marker Gene Expression
  • 07:22Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里, 我们提出了一个协议, 用于构建一个细胞培养室, 旨在将细胞暴露在各种类型的电刺激中, 并将其用于治疗间充质干细胞, 以增强成骨分化。

Tags

Electrical Stimulation ChamberMesenchymal Stem CellsOsteogenic DifferentiationCell BehaviorPlatinum WireSilver-coated WireLEDSix-well Plate LidTerminal Block Connector

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