Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Flow Cytometric Analysis of Extracellular…
JoVE Journal
Biology

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Biology
Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media
 

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

Article DOI: 10.3791/59128-v • 08:32 min • February 12th, 2019
February 12th, 2019 •
*1, *1, 1,2,3, 1
1Cellular and Molecular Cardiology Laboratory, Cardiocentro Ticino Foundation, Switzerland, 2Molecular Cardiology Institute, Dept. of Cardiology, University of Zürich, 3Faculty of Biomedical Science, Università Svizzera Italiana, Switzerland
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

The protocol describes a reproducible method designed for use with cell culture supernatants to detect surface epitopes on small extracellular vesicles (EV). It utilizes specific EV immunoprecipitation using beads coupled with antibodies that recognize surface antigen CD9, CD63, and CD81. The method is optimized for downstream flow cytometry analysis.

Tags

Flow Cytometric Analysis Extracellular Vesicles Cell-conditioned Media Diagnostic Biomarker Therapeutic Tool Flow Cytometric Method Characterizing Markers Assized Particles Conventional Seismometers Blood Arrived AVs Tissue Biopsies Set-up Method Section Petri-dishes Porcine Skin Gelatin Cardiac Progenitor Cells Iscove's Modified Dulbecco's Medium Fetal Bovine Serum Penicillin And Streptomycin Incubation 80% Confluency Dulbecco's PBS Serum-free Exosome Production Medium
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter