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细胞调理介质细胞外囊泡的流式细胞学分析

JoVE Journal
Biologia

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JoVE Journal Biologia

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

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细胞调理介质细胞外囊泡的流式细胞学分析

DOI:

10.3791/59128-v

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08:32 min

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February 12, 2019

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Carolina Balbi*¹, Sara Bolis*¹, Giuseppe Vassalli^2,3, Lucio Barile

¹Cellular and Molecular Cardiology Laboratory,Cardiocentro Ticino Foundation, Switzerland, ²Molecular Cardiology Institute, Dept. of Cardiology,University of Zürich, ³Faculty of Biomedical Science,Università Svizzera Italiana, Switzerland

Capitoli

00:04Titolo
01:05Conditioned Medium Collection and Processing
02:45Nanoparticle Tracking Analysis (NTA) Quantification
03:27Sample Preparation
04:17Sample Acquisition and Analysis
06:32Results: Representative Flow Cytometric Analyses of Extracellular Vesicles from Cell-conditioned Medium
07:58Conclusion

Summary

Traduzione automatica

该协议描述了一种可重复的方法, 该方法设计用于细胞培养上清液, 以检测小细胞外囊泡 (ev) 上的表面表位。它利用特定的 ev 免疫沉淀使用珠子与抗体结合, 识别表面抗原 cd9, cd63 和 cd81。该方法对下游流式细胞仪分析进行了优化。

Tags

Keywords: Flow Cytometry Extracellular Vesicles Cell-conditioned Media Biomarkers Therapeutic Tools Diagnostic Particle Characterization Surface Markers Cardiac Progenitor Cells Exosome Production Ultracentrifugation Nanoparticle Tracking Analysis

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