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Extraction of Extracellular Vesicles from Whole Tissue
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JoVE Journal Ricerca sul cancro
Extraction of Extracellular Vesicles from Whole Tissue

Extraction of Extracellular Vesicles from Whole Tissue

DOI:
10.3791/59143-v

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09:03 min

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February 07, 2019

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, ,
1Department of Biomedical Sciences,Florida State University College of Medicine

Capitoli

  • 00:04Titolo
  • 00:51Tissue Dissociation and Differential Centrifugation
  • 03:15Density Gradient Purification
  • 05:53Lysis and Immunoblot Confirmation of EV Proteins
  • 07:10Results: Analysis of Extracted Extracellular Vesicles
  • 08:09Conclusion

Summary

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Here, we provide a detailed protocol to isolate small extracellular vesicles (EVs) from whole tissues, including brain and tumor specimens. This method offers a reproducible technique to extract EVs from solid tissue for further downstream analyses.

Tags

Extracellular VesiclesUltracentrifugationEV IsolationTumor SpecimensProtease InhibitorsCentrifugationFiltrationResuspension

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