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A Versatile Method for Mounting Arabidopsis Leaves for Intravital Time-lapse Imaging
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Biologia
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JoVE Journal Biologia
A Versatile Method for Mounting Arabidopsis Leaves for Intravital Time-lapse Imaging

A Versatile Method for Mounting Arabidopsis Leaves for Intravital Time-lapse Imaging

DOI:
10.3791/59147-v

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07:52 min

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February 11, 2019

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, ,
1Japan Science and Technology Agency (JST), PRESTO, 2Department of Biological Sciences, Graduate School of Science,The University of Tokyo, 3Faculty of Life and Environmental Sciences,University of Tsukuba, 4Microbiology Research Center for Sustainability,University of Tsukuba

Capitoli

  • 00:04Titolo
  • 00:28Preparation of Plants and Pathogens
  • 01:48Pathogen Inoculation
  • 03:10Mounting the Inoculated Leaf
  • 05:02Microscopic Time-lapse Observation
  • 06:19Results: Intravital Time-lapse Imaging of Mounted Arabidopsis Leaves
  • 07:16Conclusion

Summary

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Traduzione automatica

We report a simple and versatile method for performing fluorescent live-imaging of Arabidopsis thaliana leaves over an extended period of time. We use a transgenic Arabidopsis plant expressing a fluorescent reporter gene under the control of an immunity-related promoter as an example for gaining spatiotemporal understanding of plant immune responses.

Tags

Arabidopsis LeavesIntravital Time-lapse ImagingSpatiotemporal RegulationGene Of InterestImmunityPromoter ActivityPathogen InoculationBacterial SuspensionFluorescence StereomicroscopePR1 Promoter

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