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En positionerings anordning til placering af mus under intranasal siRNA-levering til central nervesystemet
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
A Positioning Device for the Placement of Mice During Intranasal siRNA Delivery to the Central Nervous System

En positionerings anordning til placering af mus under intranasal siRNA-levering til central nervesystemet

DOI:
10.3791/59201-v

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05:28 min

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August 15, 2019

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, , , ,
1Department of Bioengineering and Institute of Nanoscience and Technology,Hanyang University, 2Department of Internal Medicine, Section of Infectious Diseases,Yale University School of Medicine

Capitoli

  • 00:04Titolo
  • 00:28Mouse Positioning
  • 01:23Intranasal Delivery
  • 03:28Results: Distribution of Fluorescently Labeled Peptide and siRNA after Intranasal Administration
  • 04:52Conclusion

Summary

Traduzione automatica

Traduzione automatica

Her præsenterer vi en protokol ved hjælp af en mus positionering enhed, der gør det muligt for passende placering af mus til intranasal administration af en hjerne-målretning peptid-siRNA formulering muliggør effektiv genhæmning i centralnervesystemet.

Tags

Keywords: Positioning DeviceIntranasal DeliveryCentral Nervous SystemSiRNAMiceAnesthesiaIntranasal InoculationRVG9R SiRNA ComplexMicropipetteReproducibility

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