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哺乳动物细胞周期内蛋白质激酶 rna 激活的 rna 相互作用研究
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
DOI:

10:05 min

March 05, 2019

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Capitoli

  • 00:04Titolo
  • 01:24Preparation of S or M Phase-arrested Cells
  • 02:19Formaldehyde Cross-linking
  • 04:49Immunoprecipitation
  • 07:32Results: Capturing and Analyzing PKR-interacting RNAs During the Cell Cycle Using qRT-PCR
  • 08:45Conclusion

Summary

Traduzione automatica

我们提出了利用 hela 细胞研究哺乳动物细胞周期中双链 rna 结合蛋白激酶 rna 激活 (pkr) 的 rna 相互作用的实验方法。该方法利用甲醛对交联 rna-pkr 复合物和免疫沉淀进行富集 pkr 约束 rna。这些 rna 可以通过高通量测序或 qrt-pcr 进一步分析。

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