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使用开源 MALDI TOF-MS IDBac 管道分析微生物蛋白和专用代谢物数据
JoVE Journal
Biochimica
This content is Free Access.
JoVE Journal Biochimica
Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data
DOI:

09:29 min

May 15, 2019

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Capitoli

  • 00:04Titolo
  • 01:10Preparation of MALDI Target Plates and Data Acquisition
  • 02:02Installing the IDBac Software and Starting with Raw Data
  • 02:47Work with Previous Experiments
  • 03:22Setting up Protein Data Analysis and Creating Mirror Plots
  • 04:07Clustering Samples Using Protein Data
  • 04:49Customizing the Protein Dendrogram and Inserting Samples from a Separate Experiment into the Dendrogram
  • 05:32Analyzing Specialized Metabolite Data and Metabolite Association Networks (MANs)
  • 06:51Results: Analysis of Microbial Protein and Specialized Metabolite Data by IDBac
  • 08:28Conclusion

Summary

Traduzione automatica

IDBac 是一种基于开源质谱学的生物信息学管道,它整合了从完整蛋白质和专用代谢物光谱中收集的数据,这些数据来自从细菌菌落中刮取的细胞材料。该管道使研究人员能够迅速将数百到数千个细菌菌落组织成假定的分类组,并根据专门的代谢物生产进一步区分它们。

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