/
/
Patient-derived Orthotopic Xenograft Models for Human Urothelial Cell Carcinoma and Colorectal Cancer Tumor Growth and Spontaneous Metastasis
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:04Titolo
- 00:55Tumor Enzymatic Digestion
- 02:54UCC Mouse Model
- 04:48CRC Mouse Model
- 06:02Bioluminescent Imaging, Organ Harvesting and Evaluation
- 06:55Results: Xenograft Tumor Analysis and Comparison with Primary Patient Tumors
- 08:44Conclusion
This protocol describes the generation of patient-derived orthotopic xenograft models by intra-vesically instilling high-grade urothelial cell carcinoma cells or intra-rectally injecting colorectal cancer cells into non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice for primary tumor growth and spontaneous metastases under the influence of lymph node stromal cells, which mimics the progression of human metastatic diseases.
