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在在线 MRI 图像的指导下,对流增强将视遗传阿德诺相关病毒载体输送到 Rhesus Macaque 的皮质

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images

Please note that all translations are automatically generated. Click here for the English version.

在在线 MRI 图像的指导下,对流增强将视遗传阿德诺相关病毒载体输送到 Rhesus Macaque 的皮质

DOI:

10.3791/59232-v

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08:52 min

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May 23, 2019

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Karam Khateeb², Devon J. Griggs³, Philip N. Sabes, Azadeh Yazdan-Shahmorad^2,3,4

¹Departments of Bioengineering,University of Washington, ²Washington National Primate Research Center, ³Department of Electrical and Computer Engineering,University of Washington, ⁴Department of Physiology and Center for Integrative Neuroscience,University of California, San Francisco

Capitoli

00:04Titolo
00:39MR-compatible Chamber Implantation
02:05Viral Vector Delivery
06:18Results: Validation of Viral Expression
07:47Conclusion

Summary

Traduzione automatica

在这里,我们演示了磁共振 (MR) 引导的对流增强病毒载体到皮层 (CED) 到皮层作为一种有效和简化的方法,以实现光遗传学表达在大型皮质区域在猴脑。

Tags

Convection Enhanced Delivery Optogenetic Adeno-associated Viral Vector Cortex Rhesus Macaque MRI Craniotomy Dura MR Compatible Chamber Injection Grid Saline Sterile Absorbable Gel Foam Vitamin E Capsule

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