È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:04Titolo
- 02:01Plasmid Design and Construction
- 03:20HEK-293T Cells Culturing, Plating, and Transfection
- 05:15Virus Harvesting and Viral Particles Concentrating
- 08:16Transduction of MD NPCs
- 09:48Analysis of Methylation Changes
- 11:04Results: Assessment of the LV-dCas9-DNMT3A-GFP/Puro Vectors Compared to the Naive GFP Counterpart
- 12:45Conclusion
