/

Immunologia e infezioni

/

体内自身免疫性脑髓炎实验性神经血管内障碍和胶质屏障的可视化

JoVE Journal
Immunologia e infezioni

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Immunologia e infezioni

Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo

Please note that all translations are automatically generated. Click here for the English version.

体内自身免疫性脑髓炎实验性神经血管内障碍和胶质屏障的可视化

DOI:

10.3791/59249-v

•

10:50 min

•

March 26, 2019

•

Silvia M. Tietz, Britta Engelhardt

¹Theodor Kocher Institute,University of Bern

Capitoli

00:04Titolo
00:53Immunization of C57BL/6 Mice
02:04Scoring of EAE Mice
03:08In Vivo Permeability Assay
04:25Dissection and Freezing of Brains
06:15In Situ Zymography Combined with Laminin/CD45 Immunofluorescence Staining
08:21Results: Course of EAE and In Vivo Permeability
09:42Conclusion

Summary

Traduzione automatica

在这里, 我们提出的方案, 以调查损伤的神经血管单位在实验性自身免疫性脑髓炎在体内。我们专门讨论了如何确定血液脑屏障通透性和明胶酶活性与白细胞迁移跨越胶质细胞限制。

Tags

Experimental Autoimmune Encephalomyelitis Blood-brain Barrier Matrix Metalloproteinases Immune Cell Infiltration Neuroinflammation EAE Scoring Mouse Model MOG Peptide Freund’s Adjuvant Pertussis Toxin

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

鼠标垂体免疫原的制备诱导实验性自身免疫性垂体炎

SJL小鼠诱导实验性自身免疫性垂体炎

在实验性自身免疫性脑脊髓炎（EAE）抗性品系小鼠继转移和抗原挑战克服反应迟钝

髓鞘少突胶质细胞糖蛋白（MOG<sub> 35-55</sub>）诱导实验性自身免疫性脑脊髓炎（EAE）的C57BL / 6小鼠

在免疫细胞在不同组织与疾病相关的分布和小鼠实验评价性自身免疫性脑脊髓炎的诱导

可视化痰生物膜发展影响使用腔室盖玻片型号

实验自身免疫性脑脊髓炎中枢神经系统淋巴细胞渗透的流量细胞计量分析

一种快速、简单、标准化的均质化制备抗原/辅助乳剂以诱导实验性自身免疫性脑脊髓炎的方法

副结核分枝杆菌增强实验性自身免疫性脑脊髓炎期间自身抗原免疫原的辅助活性

对实验性自身免疫性脑脊髓炎中的中枢神经系统炎症、脱髓鞘和轴突损伤进行评分

Read Article