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Immunologia e infezioni

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脂肪组织中的血管和神经纤维的共染色

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue

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脂肪组织中的血管和神经纤维的共染色

DOI:

10.3791/59266-v

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12:05 min

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February 13, 2019

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Xin Li, Zhengmei Mao, Li Yang, Kai Sun

¹Center for Metabolic and Degenerative Diseases, the Brown Foundation of Institute of Molecular Medicine,University of Texas Health Science Center at Houston, ²Microscopy Core, the Brown Foundation of Institute of Molecular Medicine,University of Texas Health Science Center at Houston

Capitoli

00:04Titolo
00:53Adipose Tissue Collection and Antibody Incubation
05:17Image Acquisition and Analysis of Blood Vessel and Nerve Fiber Networks
09:37Results: Whole Mount Co-immunofluorescence Staining of Blood Vessels and Nerve Fibers in Adipose Tissues
11:17Conclusion

Summary

Traduzione automatica

新的血管形成和交感神经支配在脂肪组织重塑中起着举足轻重的作用。然而, 在对脂肪组织进行可视化和定量测量方面仍然存在技术问题。在这里, 我们提出了一个协议, 以成功地标记和定量比较血管和神经纤维在不同的脂肪组织的密度。

Tags

Angiogenesis Sympathetic Innervation Blood Vessels Nerve Fibers Immunofluorescence Confocal Microscopy Fixation Permeabilization Blocking Primary Antibodies Endomucin Tyrosine Hydroxylase

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