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体外肿瘤细胞恢复对 chimeric 抗原受体 t 细胞抗肿瘤功能的预测评价

JoVE Journal
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JoVE Journal Ricerca sul cancro

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function

Please note that all translations are automatically generated. Click here for the English version.

体外肿瘤细胞恢复对 chimeric 抗原受体 t 细胞抗肿瘤功能的预测评价

DOI:

10.3791/59275-v

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08:04 min

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February 27, 2019

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Dongrui Wang², Renate Starr, Darya Alizadeh, Xin Yang, Stephen J. Forman*¹, Christine E. Brown*¹

¹Department of Hematology & Hematopoietic Cell Transplantation, T Cell Therapeutics Research Laboratory,City of Hope Beckman Research Institute and Medical Center, ²Irell and Manella Graduate School of Biological Sciences,City of Hope Beckman Research Institute and Medical Center

Capitoli

00:04Titolo
00:54Tumor Cell: T Cell Co-culture Setup and Tumor Cell Rechallenge
02:37Sample Harvest for Flow Cytometric Analysis
04:12Functional and Phenotypic Chimeric Antigen Receptor (CAR) T Cell Analysis
05:14Results: Representative CD4+ and CD8+ CAR T Cell Phenotypes After Repetitive Challenge
07:30Conclusion

Summary

Traduzione automatica

在这里, 我们描述了一种体外共培养方法, 递归挑战肿瘤靶向 t 细胞, 允许表型和功能分析抗肿瘤 t 细胞活性。

Tags

In Vitro Tumor Cell Rechallenge Chimeric Antigen Receptor CAR T Cells Anti-tumor Function Glioblastoma Tumorspheres Co-culture High Throughput Screening Cell Dissociation Cell Suspension Cell Culture Supernatant Trypsin EDTA

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