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キメラ抗原受容体の T 細胞の抗腫瘍機能の予測評価のため培養腫瘍細胞の再投与に
JoVE Journal
Ricerca sul cancro
This content is Free Access.
JoVE Journal Ricerca sul cancro
In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function
DOI:

08:04 min

February 27, 2019

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Capitoli

  • 00:04Titolo
  • 00:54Tumor Cell: T Cell Co-culture Setup and Tumor Cell Rechallenge
  • 02:37Sample Harvest for Flow Cytometric Analysis
  • 04:12Functional and Phenotypic Chimeric Antigen Receptor (CAR) T Cell Analysis
  • 05:14Results: Representative CD4+ and CD8+ CAR T Cell Phenotypes After Repetitive Challenge
  • 07:30Conclusion

Summary

Traduzione automatica

ここでは、抗腫瘍 T 細胞の活動の表現型および機能解析を可能にする T 細胞を腫瘍をターゲットとした再帰的に挑戦、体外培養法について述べる。

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