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In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette
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Neuroscienze
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JoVE Journal Neuroscienze
In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette

In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette

DOI:
10.3791/59286-v

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10:54 min

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June 07, 2019

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, , , , , ,
1Department of Neuroscience, Faculty of Medicine and Health Science,University of Copenhagen, 2Department of Clinical Neurophysiology,Rigshospitalet

Capitoli

  • 00:04Titolo
  • 00:57Surgical Procedure: Tracheotomy
  • 01:55Surgical Procedure: Catheter Insertion
  • 02:59Surgical Procedure: Craniotomy
  • 05:29Two-photon Imaging
  • 06:32Insertion of the Glass Micro-pipette
  • 09:11Results: ATP Puffing by Micro-pipette Induces Vessel Dilation, followed by Constriction
  • 10:30Conclusion

Summary

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We present an optimized local ejection procedure using a glass micro-pipette and a fast two-photon hyperstack imaging method, which allows precise measurement of capillary diameter changes and investigation of its regulation in three dimensions.

Tags

Three-dimensionalTwo-photon MicroscopyConducted Vascular ResponsesATP EjectionGlass Micro-pipetteCerebral CapillariesCerebral Blood Flow RegulationAstrocytic Calcium Responses

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