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黄荧光蛋白标记 p62 在攻击样诱导结构中光漂白后的荧光恢复

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures

Please note that all translations are automatically generated. Click here for the English version.

黄荧光蛋白标记 p62 在攻击样诱导结构中光漂白后的荧光恢复

DOI:

10.3791/59288-v

•

12:58 min

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March 26, 2019

•

David J. Rademacher, Maleen Cabe, Joanna C. Bakowska

¹Core Imaging Facility and Department of Microbiology and Immunology,Loyola University Chicago, ²Department of Molecular Pharmacology and Therapeutics,Loyola University Chicago

Capitoli

00:04Titolo
02:10Cell Treatments and Laser Setup
03:44Image Acquisition Setup and Collection
05:54Data Analysis
08:08Data Analysis in a Spreadsheet Program
10:16Results: Fluorescence Recovery After Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures
11:20Conclusion

Summary

Traduzione automatica

我们描述了一种全面和实用的方法, 荧光恢复后的光漂白实验与活细胞。虽然该协议被用来测量黄色荧光蛋白标记 p62 在类似侵犯诱导结构中的流动性, 但它可以应用于各种显微镜系统和荧光蛋白。

Tags

Keywords: Fluorescence Recovery After Photobleaching FRAP Yellow Fluorescent Protein P62 Aggresome-like Induced Structures Cell Biology Protein Mobility Drug Delivery Diffusion Live Cell Imaging Microscopy RAW264.7 Macrophages Lipopolysaccharide Nocodazole Tyrode’s Buffer 514 Nm Laser

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