Rapid Lipid Droplet Isolation Protocol Using a Well-established Organelle Isolation Kit

Jascha Brettschneider; Jason  M. Correnti; Chelsea Lin; Bianca Williams; Amanke Oranu; Amy Kuriakose; Dru McIver-Jenkins; Abigail Haba; Isabelle Kaneza; Sookyoung Jeon; Eleonora Scorletti; Rotonya  M. Carr

doi:10.3791/59290

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Biologia

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Rapid Lipid Droplet Isolation Protocol Using a Well-established Organelle Isolation Kit

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Biologia

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JoVE Journal Biologia

Rapid Lipid Droplet Isolation Protocol Using a Well-established Organelle Isolation Kit

DOI:

10.3791/59290-v

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08:43 min

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April 19, 2019

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Jascha Brettschneider*¹, Jason M. Correnti*¹, Chelsea Lin, Bianca Williams, Amanke Oranu, Amy Kuriakose, Dru McIver-Jenkins, Abigail Haba, Isabelle Kaneza, Sookyoung Jeon, Eleonora Scorletti, Rotonya M. Carr

¹Division of Gastroenterology,Perelman School of Medicine, University of Pennsylvania

Capitoli

00:04Titolo
00:54Tissue Preparation
03:10Lipid Droplet (LD) Isolation
05:18LD Washing
06:38Results: Analysis of LDs after Isolation
08:19Conclusion

Summary

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This protocol establishes a novel method of lipid droplet isolation and purification from mouse livers, using a well-established endoplasmic reticulum isolation kit.

Rapid Lipid Droplet Isolation Protocol Using a Well-established Organelle Isolation Kit

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