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近红外标记剂对大鼠尾静脉注射的新的最佳验证
JoVE Journal
Medicina
This content is Free Access.
JoVE Journal Medicina
A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents
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近红外标记剂对大鼠尾静脉注射的新的最佳验证

DOI:
10.3791/59295-v

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04:19 min

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April 19, 2019

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, , , , ,
1Bayer School of Natural and Environmental Sciences,Duquesne University, 2Department of Biological Sciences,Duquesne University, 3Chronic Pain Research Consortium,Duquesne University, 4Graduate School of Pharmaceutical Sciences,Duquesne University

Capitoli

  • 00:04Titolo
  • 00:47Near-infrared (NIRF)-containing Agent Tail Vein Injection
  • 03:02Results: Representative NIRF-based Nanoemulsion Tail Vein Imaging
  • 03:40Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里, 我们提出了一种方法来验证尾巴静脉注射在大鼠利用近红外荧光成像数据从染料纳入剂或生物探针。对注射前后的尾巴进行成像, 对荧光信号进行量化, 并对注射质量进行评估。

Tags

Tail Vein InjectionNear-infrared-labeled AgentsFluorescence ImagingAnesthetized AnimalTail VasculatureLateral Tail Vein25-27 Gauge NeedleNeedle PlacementSyringe InjectionCatheterBlood FlashbackQuality ControlAnimal StudiesReproducibility

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