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Generation av 3D hud Organoid från sladd blod-derived inducerade pluripotenta stamceller
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1CiSTEM Laboratory, Catholic Induced Pluripotent Stem Cell (iPSC) Research Center, College of Medicine,The Catholic University of Korea, 2Department of Biomedicine & Health Science, Seoul St. Mary’s Hospital, College of Medicine,The Catholic University of Korea, 3Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary’s Hospital, College of Medicine,The Catholic University of Korea
Capitoli
- 00:04Titolo
- 00:39Embryonic Body (EB) Generation
- 02:39Cord Blood Mononuclear Cell-derived-induced Pluripotent Stem Cell (CBMC-iPSC) Keratinocyte Differentiation
- 04:17CBMC-iPSC Fibroblast Differentiation
- 05:503D Skin Organoid Generation
- 08:00Results: Representative CBMC-iPSC-derived 3D Skin Organoid Formation
- 09:10Conclusion
Vi föreslår ett protokoll som visar hur att skilja inducerade pluripotenta stamceller-derived keratinocyter och fibroblaster och generera en 3D hud organoid, använda dessa keratinocyter och fibroblaster. Detta protokoll innehåller ytterligare ett steg för att generera en humaniserade möss modell. Tekniken presenteras här kommer förbättra Dermatologisk forskning.
