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嗜酸性粒植物视网膜免疫组织化学、西方分析和 Rna 分离的解剖

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JoVE Journal Biologia dello sviluppo

Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation

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嗜酸性粒植物视网膜免疫组织化学、西方分析和 Rna 分离的解剖

DOI:

10.3791/59299-v

•

08:47 min

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March 15, 2019

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Miles W. DeAngelis, Ruth I. Johnson

¹Department of Biology,Wesleyan University

Capitoli

00:04Titolo
00:29Eye-Brain Complex Dissection
02:09Tissue Processing for Immunofluorescence
04:56Tissue Processing for Western Blotting
06:07Tissue Processing for RNA Extraction
07:07Results: Representative Pupal Eye Protein and Gene Expression Analyses
08:28Conclusion

Summary

Traduzione automatica

本文介绍了一种解剖嗜酸性粒体木偶视网膜的手术方法, 以及免疫组织化学、西方分析和 rna 提取组织处理的方案。

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Drosophila Pupal Retina Immunohistochemistry Western Analysis RNA Isolation Dissection Eye-brain Complex Microdissection Forceps PBS Fixative

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