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Neuroscience

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JoVE Journal Neuroscience
Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation
 

Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation

Article DOI: 10.3791/59305-v • 06:50 min • August 28th, 2019
August 28th, 2019 •
1,2, 1,2, 1,2, 1,2
1Division of Biological Sciences, University of California, San Diego, 2Sanford Consortium for Regenerative Medicine

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This protocol describes a detailed procedure for resuspending and culturing human stem cell derived neurons that were previously differentiated from neural progenitors in vitro for multiple weeks. The procedure facilitates imaging-based assays of neurites, synapses, and late-expressing neuronal markers in a format compatible with light microscopy and high-content screening.

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Keywords: Human Pluripotent Stem Cells Neurons High-content Screening Neuritogenesis Synapse Maturation Replating Protease Cell Culture Dissociation Titration Centrifugation
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