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Billedbehandling protokol for analysen af Diffusion og Cluster størrelse af membranreceptorer af Fluorescens mikroskopi
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy
DOI:

12:15 min

April 09, 2019

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Capitoli

  • 00:04Titolo
  • 01:37Preparation of Biological Samples and Image Acquisition
  • 02:57Selection of Images and Creation of Masks
  • 05:45Tracking Particles
  • 08:09Calculation of the Diffusion Coefficients and Classification of Trajectories
  • 09:10Calculation of Cluster Size
  • 09:50Results: Analysis of Dynamic Fluorescence Particle Characteristics
  • 11:24Conclusion

Summary

Traduzione automatica

Vi præsenterer her, en protokol for enkelt partikel tracking billedanalyse, der giver mulighed for kvantitative evaluering af diffusion koefficienter, typer af motion og klynge størrelser af én partikler opdaget af Fluorescens mikroskopi.

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