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识别影响培养哺乳动物细胞在低到中度通量下细胞生长和生存的化合物的策略

JoVE Journal
Biochimica

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JoVE Journal Biochimica

A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

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识别影响培养哺乳动物细胞在低到中度通量下细胞生长和生存的化合物的策略

DOI:

10.3791/59333-v

•

00:12 min

•

September 22, 2019

•

Nasir Malik, Rohini Manickam, Muznabanu Bachani, Joseph P. Steiner

¹National Institute of Neurological Disorders and Stroke (NINDS), Neurotherapeutic Development Unit (NTDU),National Institutes of Health (NIH)

Capitoli

00:04Titolo
00:58Dissociate and Plate Neural Stem Cells
03:12Treating Cells with Compounds
06:10Imaging Cells on a Plate Reader
08:27Terminal MTT (3-(4,5-dimethylthizol-2-yl)-2,5-diphenyltetrazolium bromide) Cytotoxicity Assay
10:04Results: tdTomato Assay and MTT Assay
11:40Conclusion

Summary

Traduzione automatica

通常有必要评估培养细胞中一组化合物的潜在细胞毒性。在这里，我们描述了一种以 96 井格式可靠地筛选有毒化合物的策略。

Tags

Cell Growth Cell Survival Cell Culture Compound Screening Cytotoxicity Cell Proliferation Cell Viability High-throughput Low-to-moderate Throughput Multi-assay Strategy Cell Dissociation Cell Counting 96-well Plate Extracellular Matrix Coating Cell Culture Incubation

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