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Gamete Collection and In Vitro Fertilization of Astyanax mexicanus
JoVE Journal
Biologia
Author Produced
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JoVE Journal Biologia
Gamete Collection and In Vitro Fertilization of Astyanax mexicanus
DOI:

10:52 min

May 25, 2019

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Capitoli

  • 00:00Titolo
  • 01:37Light Cycle Manipulation
  • 02:25Adjusted Photoperiod and Priming Fish for Gamete Collection
  • 04:09Female Gamete Collection
  • 05:39Male Gamete Collection
  • 07:13In-vitro Fertilization
  • 08:22Risultati
  • 10:17Conclusion

Summary

Traduzione automatica

In vitro fertilization is a commonly used technique with a variety of model organisms to maintain lab populations and produce synchronized embryos for downstream applications. Here, we present a protocol that implements this technique for different populations of the Mexican tetra fish, Astyanax mexicanus.

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