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Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
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Biochimica
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JoVE Journal Biochimica
Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

DOI:
10.3791/59336-v

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09:50 min

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August 14, 2019

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, , , , , , , ,
1College of Bioscience and Biotechnology,Yangzhou University, 2Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China,Yangzhou University, 3Joint International Research Laboratory of Agriculture & Agri-Product Safety,Yangzhou University, 4Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, 5The Testing Center,Yangzhou University

Capitoli

  • 00:04Titolo
  • 01:13Produce a Flavonol from a Flavanone in an in vitro Bienzyme Synthetic System
  • 02:48Thin Layer Chromatography (TLC) Analysis
  • 05:21High Performance Liquid Chromatography (HPLC) and Liquid Chromatography/ Mass Spectrometry (LC/MS) Analyses
  • 07:50Results: Conversion of Flavanone into Flavono
  • 09:16Conclusion

Summary

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The derivation of a flavonol is crucial for its application in healthcare and the food industry. Here, we provide a detailed protocol for the biosynthesis of a flavonol from a flavanone and discuss the crucial steps and its advantages over other approaches.

Tags

Keywords: Flavonol BiosynthesisFlavanoneOne-pot Bienzymatic CascadeRecombinant EnzymesSecondary Metabolite ProductionSynthetic BufferFlavonoid Stock SolutionTLC Analysis

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